| Method |
P.493 left column 2nd paragraph: "Algal genome sizes were derived from either complete genome sequence data or nuclear DNA content estimates. Viral genome sizes were derived from either complete
genome sequences or estimates derived from electrophoretic separations of viral nucleic acids. Burst sizes were derived from either indirect estimates from dilution to-extinction assays of infectivity (Suttle, 1993) or direct
counts done with flow cytometry or transmission electron microscopy (TEM). Whenever possible, researchers used direct count estimates, since viral burst size estimates based on infectivity can underestimate viral particle numbers (Van Etten, 1983 Cotrell & Suttle, 1995). The majority of burst size data reports from the literature were not accompanied by full data sets or statistical analyses. In most instances, ranges of burst sizes were reported. Researchers therefore plotted either the mean values (when provided) or the midpoints of the ranges, as estimates of central tendency for burst size. Alternate plots using maximum and minimum burst estimates did not substantially alter the correlations
observed (data not shown). Data were log transformed prior to plotting to better accommodate a four order of magnitude range of values for burst size, and to minimize excess influence on regression plots from large viral bursts relative to small bursts, as determined using SYSTAT (Systat Software Inc., Richmond CA)." |