| Value |
34.56
umole Pi/mg protein
|
| Organism |
Corn Zea mays |
| Reference |
J. W. RIP and W. E. RAUSER, PARTIAL PURIFICATION AND SOME PROPERTIES OF ALKALINE INORGANIC PYROPHOSPHATASE , Phytochemistry1, 971, Vol. 10, pp. 2615 to 2619 Table 1 Table - link |
| Method |
DEAE (Diethylaminoethyl)cellulose
chromatography: Alkaline inorganic pyrophosphatase activity was eluted as a single peak from a DEAE cellulose
column. The leading and trailing 8 ml fraction represented 4.5 and 9.1 per cent
of the activity in the single peak fraction respectively (Table 1). |
| Comments |
umole Pi/mg Protein means umole Pi/mg Pyrophosphatase. The behaviour of corn alkaline inorganic pyrophosphatase on (NH4)2S04 fractionation
resembled human erythrocyte pyrophosphatase in that the bulk of the enzyme was recovered
in the 0.35-0.53 g/ml starting volume fraction. Corn alkaline inorganic pyrophosphatase
differed markedly however, from potato pyrophosphatase where 65 per cent was recovered
in the 0.224-0.448 g/ml starting volume fraction. |
| Entered by |
Uri M |
| ID |
104835 |