Metal ion activation of partially purified corn alkaline inorganic pyrophosphatase

Range Table - link
Organism Corn Zea mays
Reference J. W. RIP and W. E. RAUSER, PARTIAL PURIFICATION AND SOME PROPERTIES OF ALKALINE INORGANIC PYROPHOSPHATASE , Phytochemistry1, 971, Vol. 10, pp. 2615 to 2619 Table 1
Method The metals were used as their chlorides in the standard assay lacking Mg2+. The enzyme preparation used was that following DEAE-cellulose column chromatography and dialysis against 1 mM mercaptoethanol, 50 mM Tris-HCl (pH 8.7). A volume equivalent to 0.26 units of pyrophosphatase activity prior to dialysis was added to each reaction tube.
Entered by Uri M
ID 104836