| Range |
15-250 picomole/OD 436 unit
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Vogel U, Pedersen S, Jensen KF. An unusual correlation between ppGpp pool size and rate of ribosome synthesis during partial pyrimidine starvation of Escherichia coli. J Bacteriol. 1991 Feb173(3):1168-74 Table - link PubMed ID1704003
|
| Method |
Escherichia coli was exposed to partial pyrimidine starvation by feeding a pyrBI strain orotate as the only pyrimidine source. Ribosome synthesis rate was determined according to prokaryotic elongation factors (EF-Tu (elongation factor thermo-unstable)) and ribosomal protein S1): Alpha (EF-Tu or Sl) means the differential rate of synthesis of that particular protein relative to the rate of total protein synthesis in arbitrary units for each protein.
Alpha was determined by growing the cells with [3H]lysine and pulse-labeling for 2 min with [35S]methionine. The nucleotide pools are expressed as picomoles per
OD436 unit. |
| Comments |
UTP level decreased from 250 to 110 to 33 picomoles per OD436 unit on decreasing Orotate media from 200 to 75 to 50 ug/ml. Table gives rRNA synthesis rates (see Measurement Method), DNA, RNA, protein, ppGpp (Guanosine tetraphosphate), and nucleoside triphosphate levels for E. coli grown on different levels of pyrimidine supply with differing Doubling time. |
| Entered by |
Uri M |
| ID |
104806 |