UTP concentration in E. coli grown on synthetic medium containing 0.1 mM phosphate

Value 2 mM Range: +-0.5 mM
Organism Bacteria Escherichia coli
Reference Danchin A, Dondon L, Daniel J. Metabolic alterations mediated by 2-ketobutyrate in Escherichia coli K12. Mol Gen Genet. 1984193(3):473-8. Table - link PubMed ID6369074
Method The strains used throughout this work were the isogenic prototrophic strains FB8 (relA +) and FB8r (relA) (Uzan and Danchin 1978). Cells were grown in a synthetic medium containing 0.1 mM phosphate as already described (Danchin and Dondon 1980) and labeled with 32P (150 microCi of carrier free, neutralized 32P. phosphate, Amersham, UK) 90 min before addition of inhibitors (Danchin and Dondon 1980). Chromatography of Phosphorylated Molecules: Small phosphorylated molecules were submitted to chromatography on PEI-cellulose sheets (Machery-Nagel, Diiren, FRG) in either one or two dimensions. Sheets were then autoradiographed and relevant spots were cut out and counted for radioactivity in a liquid scintillation counter.
Comments For value of 8.3 mM using Liquid chromatography-mass spectrometry (LC-MS/MS) in a 2009 research see BNID 104696
Entered by Uri M
ID 104820