| Value |
5.7
nmole Pi/min/ug PPi
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Kent RB, Guterman SK. Pyrophosphate inhibition of rho ATPase: a mechanism of coupling to RNA polymerase activity. Proc Natl Acad Sci U S A. 1982 Jul 79(13):3992-6. Table 3PubMed ID6125940
|
| Method |
Preparation of p and Assay of ATPase, [tritium]Poly(C) Binding Assay, Kinetic Measurements, Pyrophosphatase Assays, Protein Determination, |
| Comments |
Incubated 15 min in absence of proteins. Pi yield is expressed as nmol/min per volume comparable to that used for assays of rho transcription termination factor in E. coli and yeast pyrophosphatase (BNID 104788-90). |
| Entered by |
Uri M |
| ID |
104791 |