Cleavage rate of pyrophosphate into Pi by rho-115 protein

Value 5.7 nmole Pi/min/ug PPi
Organism Bacteria Escherichia coli
Reference Kent RB, Guterman SK. Pyrophosphate inhibition of rho ATPase: a mechanism of coupling to RNA polymerase activity. Proc Natl Acad Sci U S A. 1982 Jul 79(13):3992-6. Table 3PubMed ID6125940
Method Preparation of p and Assay of ATPase, [tritium]Poly(C) Binding Assay, Kinetic Measurements, Pyrophosphatase Assays, Protein Determination,
Comments Assay mixtures containing 0.2 ug of protein were incubated for 15 min. Note: this PPi cleavage rate is identical to rate without protein (BNID 104791), thus rho is not a pyrophosphorylase. See also BNID 104788, 104790
Entered by Uri M
ID 104789