Cleavage rate of pyrophosphate into Pi by Yeast inorganic pyrophosphatase

Value 432.3 nmole Pi/min/ug PPi
Organism Budding yeast Saccharomyces cerevisiae
Reference Kent RB, Guterman SK. Pyrophosphate inhibition of rho ATPase: a mechanism of coupling to RNA polymerase activity. Proc Natl Acad Sci U S A. 1982 Jul 79(13):3992-6. Table 3PubMed ID6125940
Method Preparation of p and Assay of ATPase, [tritium]Poly(C) Binding Assay, Kinetic Measurements, Pyrophosphatase Assays, Protein Determination,
Comments Assay mixtures containing 0.05 ug of protein were incubated for 1 or 2 min. For PPi cleavage rate in E. coli by rho transcription termination factor See BNIDs 104788-9. See also BNID 104791
Entered by Uri M
ID 104790