| Value |
432.3
nmole Pi/min/ug PPi
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Kent RB, Guterman SK. Pyrophosphate inhibition of rho ATPase: a mechanism of coupling to RNA polymerase activity. Proc Natl Acad Sci U S A. 1982 Jul 79(13):3992-6. Table 3PubMed ID6125940
|
| Method |
Preparation of p and Assay of ATPase, [tritium]Poly(C) Binding Assay, Kinetic Measurements, Pyrophosphatase Assays, Protein Determination, |
| Comments |
Assay mixtures containing 0.05 ug of protein were incubated for 1 or 2 min. For PPi cleavage rate in E. coli by rho transcription termination factor See BNIDs 104788-9. See also BNID 104791 |
| Entered by |
Uri M |
| ID |
104790 |