| Value |
230
µm^2/sec
|
| Organism |
Chicken Gallus gallus |
| Reference |
García-Pérez AI, López-Beltrán EA, Klüner P, Luque J, Ballesteros P, Cerdán S. Molecular crowding and viscosity as determinants of translational diffusion of metabolites in subcellular organelles. Arch Biochem Biophys. 1999 Feb 15 362(2):p.333 right column 2nd paragraphPubMed ID9989943
|
| Method |
"The role of molecular crowding and viscosity on the apparent translational diffusion coefficient (ADC) of small metabolites was investigated in different subcellular organelles using the pulse-field gradient spin-echo 1H NMR technique. ADCs of metabolites with increasing radius of gyration (0.7 Å < RG < 4.5 Å) were measured in the cytoplasm of rat or chicken erythrocytes, in the nucleus of chicken erythrocytes, and in isolated rat liver mitochondria. Metabolite ADCs in these systems were compared with the corresponding ADCs determined in model solutions of increasing bulk viscosity but different molecular crowding." |
| Comments |
"Optical microscopy measurements showed that nucleus and cytosol contribute 10.35±2.44% (n=48) and 91.99±4.18% (n=48) of the intracellular volume (121.47±1.29µm^3, n=9), respectively. Complementary, hematocrit measurements provided the remaining values for the extracellular space Ve. Water ADCe and ADCc (10^-5cm^2/s) determined from biexponential fits gave values of 1.81 and 0.900 (Fig. 3, Table I). Thus, an ADCn value of 2.30×10^-5cm^2/s for water in the nucleus could be calculated since the relative contributions of Vn, Vc, and Ve, as well as ADCC and ADCe are known." See also Diffusion coefficient of Water molecule in water 2100µm^2/sec (BNID 104087) and in rat liver mitochondria 580 µm^2/sec (BNID 104647) |
| Entered by |
Uri M |
| ID |
104645 |