| Value |
2850
µm^3
|
| Organism |
Human Homo sapiens |
| Reference |
Maryna Bondarava, Thesis: Hypertonicity-Induced Cation Channels in HepG2 cells: Role in Proliferation and Putative Molecular Correlates, pp. 87 Max Planck Institute of Molecular Physiology, Department of Epithelial Cell Physiology/Systemic Cell Biology, 2007 link - link |
| Primary Source |
Weiss,E.C., Wehner,F., Lemor,R.M. Measuring cell volume regulation with time resolved acoustic microscopy. Acoustical Imaging 2007 73-80. |
| Method |
After the role of a-ENaC in hypertonicity-induced membrane currents of HepG2 cells was established its actual role in cell volume regulation was of interest. Experiments were performed by the novel technique of time resolved acoustic microscopy. The major advantages of this method for cell volume measurement are the high accuracy, the absence of any radiation damage and photobleaching and the opportunity to use non-transparent samples and substrates (Weiss et al 2007, Primary source) |
| Comments |
Experiments were done under control conditions, following treatment with transfection reagent, and with cells transfected with negative control siRNA and a- ENaC-siRNA. Under the isotonic conditions (300 mosmol/l), cell volumes equaled 2,83 ± 0,24 pl (n = 31) (Figure 3.9), 3,08 ± 0,41 pl (n = 6), 2,49 ± 0,20 pl (n = 13), and 3,01 ± 0,24 pl (n = 24) in control cells. Value is the mean: [(2.83×31)+(3.08×6)+(2.49×13)+(3.01×24)]/[31+6+13+24]=2.849 pl |
| Entered by |
Uri M |
| ID |
104614 |