| Value |
1.11e-3
Sec^-1
Range: Table - link Sec^-1
|
| Organism |
Human Homo sapiens |
| Reference |
Nalefski EA, Nebelitsky E, Lloyd JA, Gullans SR. Single-molecule detection of transcription factor binding to DNA in real time: specificity, equilibrium, and kinetic parameters. Biochemistry. 2006 Nov 21 45(46):13794-806 table 2PubMed ID17105198
|
| Method |
Researchers present a novel methodology to quantify the binding of proteins to target DNA molecules based on single-molecule detection and real-time counting of individual free and bound fluorescently tagged molecules flowing past a detection device. Using this technology, they measured DNA binding by fluorescently tagged domains of four distinct transcription factors, namely, human early growth response protein Egr-1. Dissociation kinetics measured by single-molecule event counting on the Trilogy 2020 device. Representative time course of Cy5-EBS dissociation initiated by adding 2.5 nM EBS to the His-GFP-Egr ZFD prebound to Cy5- EBS (25 pM). |
| Comments |
The dissociation time course was initiated by the addition of a large molar excess of unlabeled EBS to the protein prebound to Cy5-EBS, as in Figure 9A. Data were fitted with monoexponential eq 1. |
| Entered by |
Uri M |
| ID |
104606 |