| Range |
100-200 µsec
|
| Organism |
Bacteria Thermotoga maritima |
| Reference |
Chung HS, Louis JM, Eaton WA. Feature Article: Experimental determination of upper bound for transition path times in protein folding from single-molecule photon-by-photon trajectories. Proc Natl Acad Sci U S A. 2009 Jul 10PubMed ID19584244
|
| Primary Source |
Rhoades E, Cohen M, Schuler B, Haran G. Two-state folding observed in individual protein molecules. J Am Chem Soc. 2004 Nov 17 126(45):14686-7.PubMed ID15535670
|
| Method |
(of primary source:) The cold-shock protein from Thermotoga maritima (CspTm) was labeled with a green-fluorescing and a red-fluorescing dye, serving as FRET donor and acceptor, respectively. FRET upon excitation by a laser beam allows folded and unfolded molecules to be distinguished on the basis of the strong distance dependence of energy transfer between the chromophores. |
| Comments |
Researchers' results provide an upper bound of ~200 µs for this transit time, which is in agreement with the simple assumptions made in previous analyses of protein folding. |
| Entered by |
Uri M |
| ID |
104596 |