||Grass Miscanthus giganteus
||Wang D, Portis AR Jr, Moose SP, Long SP. Cool C4 photosynthesis: pyruvate Pi dikinase expression and activity corresponds to the exceptional cold tolerance of carbon assimilation in Miscanthus x giganteus. Plant Physiol. 2008 Sep148(1):557-67PubMed ID18539777
||PPDK Extraction and Activity Assays. Immunoblot Analysis Total soluble proteins on a leaf area basis were separated by 10% Tris-Gly SDS-PAGE and blotted onto the polyvinylidene difluoride membrane. PPDK and Rubisco proteins were detected with rabbit polyclonal antibodies raised against recombinant maize PPDK (Budde and Chollet, 1986) and native spinach (Spinacia oleracea) Rubisco.
||When quantified in absolute terms, PPDK content in 25°C grown M. × giganteus leaves was 0.30 g/m2 and increased to 0.56 g/m2 after 14 d at 14°C