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Time for complete recovery of TATA Binding Protein (TBP) fluorescence signal

Value	15 sec
Organism	Budding yeast Saccharomyces cerevisiae
Reference	Sprouse RO, Karpova TS, Mueller F, Dasgupta A, McNally JG, Auble DT. Regulation of TATA-binding protein dynamics in living yeast cells. Proc Natl Acad Sci U S A. 2008 Sep 9 105(36):13304-8PubMed ID18765812
Method	To determine whether the dynamic behavior of the Pol II machinery in vivo is fundamentally different from that of Pol I and whether the static behavior of Pol II factors in vitro fully recapitulates their behavior in vivo, researchers used fluorescence recovery after photobleaching (FRAP). Signal noise resulting from the small size of the bleach spot combined with the relatively faint intranuclear YFP signals was overcome by collection of FRAP data from ˜30–100 individual cells.
Entered by	Uri M
ID	104513