| Range |
5e-4 - 4e-3 gene/PFU
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Kenzaka T, Tani K, Sakotani A, Yamaguchi N, Nasu M. High-frequency phage-mediated gene transfer among Escherichia coli cells, determined at the single-cell level. Appl Environ Microbiol. 2007 May73(10):3291-9.PubMed ID17384307
|
| Method |
Purification of bacteriophages. Sequencing of isolated phage EC10. Plaque assays. Transduction and DNA transfer experiments. Viabilities of recipient cells determined by Direct Viable Counting (DVC). (cycling primed in situ amplification-fluorescent in situ hybridization [CPRINS-FISH]). Epifluorescence microscopy. Genetic characterization of transductants. PCR amplification of the unknown region next to the (beta lactamase gene-bla) sequence on transductants and sequencing. Examination of integration of bla gene into the phage genome. |
| Comments |
PFU=Plaque Forming Unit. Transduction frequencies determined by conventional plating were <4x10^(-9) - 4x10^(-8) gene/PFU |
| Entered by |
Uri M |
| ID |
104447 |