| Value |
40
copies/cell
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Kelman Z, O'Donnell M. DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine. Annu Rev Biochem. 1995 64:171-200 p.175 2nd paragraphPubMed ID7574479
|
| Primary Source |
Maki H, Kornberg A. The polymerase subunit of DNA polymerase III of Escherichia coli. II. Purification of the alpha subunit, devoid of nuclease activities. J Biol Chem. 1985 Oct 25 260(24):12987-92PubMed ID2997151
|
| Comments |
"Core
contains
the
DNA
polymerase
and
proofreading
exonuclease activities
(primary source).
There
are
approximately
40
molecules
of
core
in
the
cell,
and
therefore
only
half
are
assembled
into
holoenzyme
(primary source).
The
three
subunits
of
core
are
tightly
associated
and
cannot
be
resolved
short
of
denaturation.
Individual
subunits
are
provided
through
use
of
the
genes." p.186 bottom line:"...there are only 10-20 molecules of holoenzymes in a cell [ref 4 PMID 6090455],..." |
| Entered by |
Uri M |
| ID |
104123 |