Number of DNA polymerase III holoenzyme molecules in cell

Range 10-20 copies/cell
Organism Bacteria Escherichia coli
Reference Kelman Z, O'Donnell M. DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine. Annu Rev Biochem. 1995 64:171-200PubMed ID7574479
Primary Source Wu YH, Franden MA, Hawker JR Jr, McHenry CS. Monoclonal antibodies specific for the alpha subunit of the Escherichia coli DNA polymerase III holoenzyme. J Biol Chem. 1984 Oct 10 259(19):12117-22PubMed ID6090455
Method Three murine hybridoma cell lines have been established that secrete monoclonal antibodies directed specifically against the alpha subunit of the DNA polymerase III holoenzyme of Escherichia coli. All three antibodies have been purified and identified to be of the IgM class. competition binding assays indicate that these antibodies bind to at least two distinct but adjacent or interacting sites. An immunoblot assay has been developed that permits quantitation of alpha in crude extracts.
Entered by Uri M
ID 104119