| Value |
22
nucleotides/sec
Range: ±3 nucleotides/sec
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Gray WJ, Midgley JE. The control of ribonucleic acid synthesis in bacteria. The synthesis and stbility of ribonucleic acid in rifampicin-inhibited cultures of Escherichia coli. Biochem J. 1971 Apr122(2):161-9PubMed ID4940607
|
| Method |
A study was made of the kinetics of labelling of the stable ribonucleic acids (rRNA+tRNA) and the unstable mRNA fraction in cultures of Escherichia coli M.R.E.600, inhibited by the addition of 0.1g of rifampicin/l. Labelling was carried out by adding either [2-(14)C]- or [5-(3)H]-uracil as an exogenous precursor of the cellular nucleic acids. From studies using DNA RNA hybridization, the kinetics of the synthesis and degradation of mRNA was followed in the inhibited cultures. |
| Comments |
Taking 2-3 min as time that is required to polymerize a complete chain of 23S rRNA, the polymerization rate of this fraction in the three media (broth, glucose-salts or lactate salts) was 25, 22 and 19 nucleotides added/s to the growing chains. |
| Entered by |
Uri M |
| ID |
104109 |