| Value |
0.3
molecules/ribosome
Range: Table - link molecules/ribosome
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Bremer, H., Dennis, P. P. (1996) Modulation of chemical composition and other parameters of the cell by growth rate. Neidhardt, et al. eds. Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 2nd ed. chapter 97 Table 4 |
| Primary Source |
Howe JG, Hershey JW. Initiation factor and ribosome levels are coordinately controlled in Escherichia coli growing at different rates. J Biol Chem. 1983 Feb 10 258(3):1954-9PubMed ID6337147
|
| Method |
Cells were grown in media containing [35S]sulfate and different carbon sources, and harvested during mid-exponential phase after about 10 generations of balanced growth. Initiation factors (IF), elongation factors (EF), and a number of ribosomal proteins were identified unambiguously in gel patterns following two-dimensional polyacrylamide gel electrophoresis. |
| Comments |
IF2 binds to an initator tRNA and controls the entry of that tRNA into the ribosome. IF2, bound to GTP, binds to the 30S P site. After associating with the 30S subunit, fMet-tRNAf binds to the IF2 then IF2 transfers the tRNA into the partial P site. When the 50S subunit joins, it hydrolyzes GTP to GMP and PPi, causing a conformational change in the IF2 that causes IF2 to release and allow the 70S subunit to form. |
| Entered by |
Uri M |
| ID |
104063 |