| Value |
800
nucleotides
Range: ±30 nucleotides
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Lee JB, Hite RK, Hamdan SM, Xie XS, Richardson CC, van Oijen AM. DNA primase acts as a molecular brake in DNA replication. Nature. 2006 Feb 2439(7076):621-4. Fig S2PubMed ID16452983
|
| Method |
in vitro, single molecule measurements. The 5' end of one strand of a 48.5 kilobase (kb)-long duplex lambda phage DNA molecule is attached to the bottom surface of a glass flow cell using a biotin–streptavidin linker. The opposite 3' end bears a digoxigenin moiety and is linked to a bead that is 2.8 microm in diameter and coated with anti-digoxigenin antibody |
| Comments |
These values are in agreement with previous ensemble studies: (1) Patel, S. S., Wong, I. & Johnson, K. A. Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. Biochemistry 30, 511-25 (1991). And (2)Tabor, S., Huber, H. E. & Richardson, C. C. Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. J Biol Chem 262, 16212-23 (1987). |
| Entered by |
Uri M |
| ID |
103996 |