| Value |
220
nucleotides/sec
Range: ±80 nucleotides/sec
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Lee JB, Hite RK, Hamdan SM, Xie XS, Richardson CC, van Oijen AM. DNA primase acts as a molecular brake in DNA replication. Nature. 2006 Feb 2 439(7076):621-4. Supplementary information p.2 bottom paragraphPubMed ID16452983
|
| Method |
in vitro, single molecule measurements. P.621 right column bottom paragraph: "The 5' end of one strand of a 48.5 kilobase (kb)-long duplex lambda phage DNA molecule is attached to the bottom surface of a glass flow cell using a biotin–streptavidin linker. The opposite 3' end bears a digoxigenin moiety and is linked to a bead that is 2.8 µm in diameter and coated with anti-digoxigenin antibody (Fig. 2a, b)." |
| Comments |
Supplementary information p.2 bottom paragraph: "The average enzymatic rate amounts to 220±80 nucleotides per second, with a processivity of 800±300 nucleotides (Fig S2). These values are in agreement with those observed in a previously published single-molecule experiment [Wuite et al, PMID 10716452, that gives polymerization rates of over 100 bases/sec for T7 DNA] and ensemble studies [PMID 1846298, PMID 3316214]." See BNID 104120 |
| Entered by |
Uri M |
| ID |
103995 |