Buoyant cell density

Value 1.1 g/ml
Organism Bacteria Escherichia coli
Reference Baldwin WW, Myer R, Powell N, Anderson E, Koch AL. Buoyant density of Escherichia coli is determined solely by the osmolarity of the culture medium. Arch Microbiol. 1995 Aug164(2):155-7 p.156 fig.3 & fig.2PubMed ID8588736
Method P.155 right column 2nd paragraph: "Escherichia coli ML308 (ATCC 15224) was used in these studies...One loopful of the overnight culture was inoculated into the appropriate osmolarity medium...NaCl was added to the Percoll solution so that density gradients were isoosmotic with the growth medium."
Comments Abstract: "[Researchers] conclude from these experiments that neither buoyant density nor chemical make-up of the medium determines the buoyant density of cells grown in that medium. Only the medium osmolarity determines cell buoyant density, suggesting that E. coli has no mechanisms to sense buoyant density." E. coli density was observed to vary from 1.09 to 1.11 g/mL in 100-500 mOsM (p.156 fig.3) Value extracted visually from fig.2 & fig.3
Entered by Uri M
ID 103875