Bend angle of A-tract (Adenine-tract) of DNA

Value 13.5 Degrees
Organism Generic
Reference Rivetti C, Walker C, Bustamante C. Polymer chain statistics and conformational analysis of DNA molecules with bends or sections of different flexibility. J Mol Biol. 1998 Jul 3280(1):41-59PubMed ID9653030
Method A conformational analysis of double-stranded DNA containing bends or an intervening single-stranded region, provides a direct application of the equations presented above. In particular, the mean-square end-to-end distance, R2, was used first to quantify the bend angle of DNA molecules possessing a number of phased A-tracts. Second, the persistence length of a single-stranded DNA segment inserted between two double-stranded fragments was determined from the R2 of the entire polymer.
Comments First observed in kinetoplast DNA [Simpson 1979 and Challberg and Englund 1980], runs of four or more adenine residues (A-tracts) introduce intrinsic bends in DNA [Crothers et al 1990]. A-tract bending has been investigated using X-ray crystallography and other solution techniques, such as gel electrophoresis, electric birefringence, circular dichroism and NMR
Entered by Uri M
ID 103795