| Reference |
Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods. 2007 Jul4(7):555-7. Supplementary table 1PubMed ID17572680
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| Method |
Photobleaching was performed in HeLa cells using Leica Sp2 confocal microscope, excitation by equalized laser lines output power (tuned to 40 mictowatts in a parked laser mode). Following parameters were used: beam expander 1 (providing high intensity of excitation light), zoom 4x, scan frequency 400 Hz, format 512x512, 1 image per 1.63 s. 458 nm laser line was used for Cerulean, 488 nm laser line for EGFP, 514 nm laser line for EYFP, 543 nm laser line for TagRFP, DsRed-Express, mCherry, and mPlum. See more details under table link |