| Range |
1.01-3.57 Table - link ng
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| Organism |
Human Homo sapiens |
| Reference |
Park K, Jang J, Irimia D, Sturgis J, Lee J, Robinson JP, Toner M, Bashir R. 'Living cantilever arrays' for characterization of mass of single live cells in fluids. Lab Chip. 2008 Jul8(7):1034-41. p.1040 table 3PubMed ID18584076
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| Primary Source |
[3] D. A. Wolff and H. Pertoft, J. Cell Biol., 1972, 55, 579–585PubMed ID4571230
|
| Method |
P.1035 left column 3rd paragraph: "One of the direct methods for measuring cell mass is the use of micromechanical resonant cantilever mass sensors for measurement and characterization of single cells. The resonance frequency of a cantilever is inversely proportional to the square root of the mass. Therefore, the mass of the entities attached to the cantilever can be directly calculated from the resonance frequency of the cantilever." |
| Comments |
P.1040 right column 2nd to bottom paragraphs: "Mass measurement: Fig. 7(a) and (b) show the measured frequency responses of the cantilevers with and without a cell in the growth media. For each case, the resonant frequency decreased by 2.93 kHz and 5.43 kHz respectively. These frequency shifts correspond to the cell mass of 1.01 ng and 3.57 ng using the procedure described in the previous section. The measured mass from the frequency response was compared with the estimated value calculated from the cell density and the cell volume. The volume of the cell was measured from the confocal microscope images by Image J (Wayne Rasband, National Institute of Health, USA), and the published value for the density of the cell was used (primary source). The measured volume of the cell for Fig. 7(a) and (b) were 2349 μm^3 and 3857 μm^3, and the resulting estimated cell masses were 2.48 ng and 4.09 ng, respectively. Table 3 shows the measured cell mass and the estimated cell mass for comparison. As shown in Table 3, the measured values and the estimated values are close to each other. One of the probable reasons for the difference was the limited growth of a cell on a cantilever, due to the cantilever's small surface area, where the cell was constrained to grow only on the cantilever." From the mean after adjustment, 2.29ng/cell, one can derive ~4.4×10^8 cells/gram. |
| Entered by |
Uri M |
| ID |
103721 |