| Range |
~7 Unitless
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Sezonov G, Joseleau-Petit D, D'Ari R. Escherichia coli physiology in Luria-Bertani broth. J Bacteriol. 2007 Dec189(23):8746-9 doi: 10.1128/JB.01368-07 abstract & p.8746 right column 5th paragraphPubMed ID17905994
|
| Method |
P.8746 right column 3rd paragraph: "The widely used wild-type E. coli K-12 strain MG1655 (ref 1), which has been sequenced and annotated (refs 2, 10), was chosen for this study. An overnight culture in Luria-Bertani broth was diluted 5,000-fold in 250 ml fresh medium in a 1-liter Erlenmeyer flask and cultivated in a shaking water bath (180 rpm) at 37°C. The OD600 and number of cells per milliliter were monitored." P.8747 right column 2nd paragraph: "...[investigators] set up a bioassay for fermentable sugars by using a hemA derivative of MG1655. This mutant is unable to synthesize δ-aminolevulinic acid. In the absence of exogenous δ-aminolevulinate, it cannot synthesize heme and thus cannot produce cytochromes, making it unable to carry out respiration. The mutant therefore absolutely requires a fermentable sugar in order to grow in the absence of δ-aminolevulinate." |
| Comments |
Abstract: "Luria-Bertani broth supports Escherichia coli growth to an optical density at 600 nm (OD600) of 7." P.8746 right column 5th paragraph: "This departure from steady-state growth is surprisingly early since the cells continue to grow for a further 20-fold increase in the mass per milliliter. As shown in Fig. 1A, the growth rate
slows down gradually from this point (an OD600 of 0.3) until saturation (an OD600 of about 7), reflecting a gradual impoverishment of the medium." |
| Entered by |
Uri M |
| ID |
103513 |