Misincorporation rate of Amino acids in translation

Range 0.001-0.0001 1/amino acid
Organism Bacteria Escherichia coli
Reference Hani S. Zaher and Rachel Green, Fidelity at the Molecular Level: Lessons from Protein Synthesis. Cell Volume 136, Issue 4, 20 February 2009 doi: 10.1016/j.cell.2009.01.036. , Pages 746-762 p.746 left column top paragraphPubMed ID19239893
Primary Source Bouadloun, F.,Donner, D., and Kurland,C.G.(1983).Codon-specific missense errors in vivo. EMBO J. 2, 1351–1356. & Edelmann, P., and Gallant, J. (1977). Mistranslation in E. coli. Cell 10, 131–137 & Kramer, E.B., and Farabaugh, P.J. (2007). The frequency of translational misreading errors in E. coli is largely determined by tRNA competition. RNA 13, 87–96. & Laughrea, M., Latulippe, J., Filion, A.M., and Boulet, L. (1987). Mistranslation in twelve Escherichia coli ribosomal proteins. Cysteine misincorporation at neutral amino acid residues other than tryptophan. Eur. J. Bioch. FEBS 169, 59–64. & Rosenberger RF, Foskett G. An estimate of the frequency of in vivo transcriptional errors at a nonsense codon in Escherichia coli. Mol Gen Genet. 1981 183(3):561-3PubMed ID10872330, 138485, 17095544, 3315664, 7038382
Method Primary source Boudalon et al. 1983 p.1351 right column 3rd paragraph: "[Researchers'] experimental strategy depends on the ability to identify and purify a polypeptide with a nominal sequence that does not contain the amino acid responsible for the missense substitution under study. As shown in article, the polypeptide can be either a whole protein or an excisable sequence within a whole protein. A second requirement of their method is the availability of a chemical reagent or enzyme that will specifically cleave the polypeptide at the particular amino acid position in the polypeptide at which the putative missense event occurs. Finally, the substituted polypeptide must be resistant to the cleavage reaction. When these conditions are met, a simple combination of radioisotopic measurements of polypeptides fractionated in polyacrylamide gels permits to estimate a specific missense error rate at a particular position in a protein."
Comments P.746 left column top paragraph: "Transcription and translation proceed with considerably lower levels of fidelity, with misincorporation rates of 1 in 10^4 and 1 in 10^3–10^4, respectively [primary sources]." This level of accuracy is lower than that of DNA replication and similar to that of transcription misincorporation rate. See BNID 105069 for LystRNAs misreading rate, BNID 103469 for aminoacylation error rate. See BNID 105718
Entered by Uri M
ID 103454