Fraction of PolyP localized at the cell surface
| Range | 20-30 % |
|---|---|
| Organism | Budding yeast Saccharomyces cerevisiae |
| Reference | Vagabov VM, Trilisenko LV, Kulaev IS. Dependence of inorganic polyphosphate chain length on the orthophosphate content in the culture medium of the yeast Saccharomyces cerevisiae. Biochemistry (Mosc). 2000 Mar65(3):349-54.PubMed ID10739478 |
| Primary Source | Vagabov, V. M. (1988) Biosynthesis of Carbohydrate Components of Yeast Cell Wall [in Russian], NTsBI Akad. Nauk SSSR, Pushchino. AND Kulaev, I. S., Vagabov, V. M., and Shabalin, Yu. A. (1987) in Phosphate Metabolism and Cellular Regulation in Microorganisms (Torriani-Gorini, A., Rothman, F. G., Silver, S., Wright, A., and Yagil, E., eds.) American Society for Microbiology, Washington, pp. 233-238. |
| Method | The yeast is first cultivated for 4 h in a complete Rider medium containing 9 mM Pi and 111 mM glucose as a carbon source. Then the cells are placed on the same medium except depleted of Pi under sterile conditions and the culture is grown during 7 h. Further, the cells are again placed on fresh complete medium, where the culture is growing for 2 and 4 h. The degree of polymerization (n) of polyP fractions was estimated from the areas of 31P-NMR-spectral peaks. |
| Comments | The content of this polyP may comprise 20-30% of the total amount of cell polyP depending on the conditions of cultivation. See BNID 103450, 105839 |
| Entered by | Uri M |
| ID | 103449 |