| Value |
16250
µg P/g dry cell weight
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Vagabov VM, Trilisenko LV, Kulaev IS. Dependence of inorganic polyphosphate chain length on the orthophosphate content in the culture medium of the yeast Saccharomyces cerevisiae. Biochemistry (Mosc). 2000 Mar65(3):349-54. p.351 Fig. 1(a)PubMed ID10739478
|
| Method |
P.350 right column 4th paragraph: "Phosphate concentration was estimated according to Berenblum and Chain in the modification of Weil-Malherbe and Green (ref 16)." P.350 right column 5th paragraph: "To determine dry weight, aliquots of cell suspensions were applied on filters preliminary brought to a constant weight by drying under vacuum at 85°C and dried to a constant weight under the same conditions." P.350 right column 8th paragraph: "The yeast is first cultivated for 4 h in a complete Rider medium containing 9 mM Pi and 111 mM glucose as a carbon source (point A)." Extracted visually from Fig. 1. a). |
| Comments |
P.351 caption to Fig1.A: "PolyP [inorganic linear polyphosphate] content in the cells of S. cerevisiae under different conditions of their cultivation: PolyP content in the cells of S. cerevisiae under different conditions of their cultivation." P.351 right column bottom paragraph: "A comparison of polyP content in the yeast before phosphate starvation (point A) and after the transfer of the culture to the fresh complete medium (point C) indicates that super-accumulation is not characteristic for all polyP fractions. As seen from Fig. 1a, the maximal accumulation under the conditions in question is observed for the polyP3 (almost 5-fold) and polyP5 (more than 8-fold) fractions." |
| Entered by |
Uri M |
| ID |
103437 |