| Value |
500
µg P/g dry cell weight
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Vagabov VM, Trilisenko LV, Kulaev IS. Dependence of inorganic polyphosphate chain length on the orthophosphate content in the culture medium of the yeast Saccharomyces cerevisiae. Biochemistry (Mosc). 2000 Mar65(3):349-54. Fig. 1(a)PubMed ID10739478
|
| Method |
The yeast is first cultivated for 4 h in a complete Rider medium containing 9 mM Pi and 111 mM glucose as a carbon source. Phosphate concentration was estimated according to Berenblum and Chain in the modification of Weil-Malherbe and Green (ref 16 of article). To determine dry weight, aliquots of cell suspensions were applied on filters preliminary brought to a constant weight by drying under vacuum at 85°C and dried to a constant weight under the same conditions. |
| Comments |
Measured manually from Fig. 1. a) PolyP content in the cells of S. cerevisiae under different conditions of their cultivation |
| Entered by |
Uri M |
| ID |
103436 |