| Value |
0.2
(nmol P)*min^-1*(mg protein)^-1
|
| Organism |
Amoeba Dictyostelium discoideum |
| Reference |
Gezelius K. Inorganic polyphosphates and enzymes of polyphosphate metabolism in the cellular slime mold Dictyostelium discoideum. Arch Microbiol. 1974 Jul 2298(4):311-29.PubMed ID4367840
|
| Method |
Extraction and Assay of Polyphosphate-ADP-Phosphotransferase. Cell-free extracts were obtained through centrifugation at 3000 g for 15 min. When tested at the culmination and sorocarp stages, ca 95% of the total activity was recovered in the supernatant.The enzyme activities were assayed at 23 degrees C in the direction of ATP |
| Comments |
P in units stands for polyphosphates. The specific activity of the polyphosphate-ADP-phosphotransferase, pH optimum 7.7, was ca 0.2 nmol Pi·min-1·mg protein-1 and rather constant during differentiation. Reaction investigated was P(n-1)+ATP>>Pn+ADP |
| Entered by |
Uri M |
| ID |
103424 |