| Value |
30
(nmol Pi)*min^-1*(mg protein)^-1
|
| Organism |
Amoeba Dictyostelium discoideum |
| Reference |
Gezelius K. Inorganic polyphosphates and enzymes of polyphosphate metabolism in the cellular slime mold Dictyostelium discoideum. Arch Microbiol. 1974 Jul 2298(4):311-29.PubMed ID4367840
|
| Method |
Extraction and Assay of Polyphosphatase. The slime mold was harvested in 0.05 35 Tris, pH 7.6, containing 2 m35 MgCl2 and 1 mM beta-mercaptoethanol. The cell suspensions were used immediately or frozen at-70degrees and stored at -20degrees the time of assay. Homogenization was performed in a French pressure cell at 1400 kg/cm2. |
| Comments |
The specific activity of the polyphosphatase, pH optimum 7.6, was ca 30 nmol Pi·min-1·mg protein-1 at all stages of development. Reaction investigated was Pn>>P(n-1)+Pi |
| Entered by |
Uri M |
| ID |
103421 |