Ki for (AMP) in ATP regeneration from AMP with ADP intermediate
| Value | 40 uM |
|---|---|
| Organism | Corn Zea mays |
| Reference | Hatch M. D., Properties and Regulation of Adenylate Kinase from Zea mays Leaf Operating in C, Pathway Photosynthesis, Aust. J. Plant Physiol., 1982, 9, 287-96 |
| Method | For the direction towards ADP formation, activity was measured as NADH oxidation at 340 nm in reactions containing enzyme, 25 mM HEPES-KOH buffer, pH 7.8 or as specified, 2 mM ATP, 0.5 mM AMP, 2.5 mM MgCl, and a coupling system consisting of 3 units of pyruvate kinase, 5 units of lactate dehydrogenase, 0.5 mM PEP, 0.25 mM NADH and 30 mM KC1 in a total volume of 1 ml. |
| Comments | AMP strongly inhibited the reverse direction reaction, acting in a strictly competitive manner with respect to ADP |
| Entered by | Uri M |
| ID | 103414 |