| Value |
30.2
sec
Range: ±4.2 sec
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Kaksonen M, Toret CP, Drubin DG. A modular design for the clathrin- and actin-mediated endocytosis machinery. Cell. 2005 Oct 21 123(2):305-20.PubMed ID16239147
|
| Method |
TIRF microscopy and epifluorescence. Researchers quantitatively analyzed the spatial and temporal localization of the C-terminal GFP fusions of each protein expressed from its endogenous locus |
| Comments |
End3-GFP initially formed an immotile patch that later moved about 200 nm toward the cell center while it was dissipating (Figures 3B and 3C). The onset of the inward movement corresponded with the arrival of Abp1-RFP at the End3-GFP patch (Figure 3D). Thus, End3p behaves like Sla1p, Pan1p, and Sla2p (Kaksonen et al., 2003). Since these proteins move like clathrin, they are likely associated with the vesicle coat. |
| Entered by |
Uri M |
| ID |
103219 |