Size of typical tether in Tethered Particle Motion method
Value | 1000 bp |
---|---|
Organism | Generic |
Reference | Garcia HG, Grayson P, Han L, Inamdar M, Kondev J, Nelson PC, Phillips R, Widom J, Wiggins PA. Biological consequences of tightly bent DNA: the other life of a macromolecular celebrity. Biopolymers. 2007 Feb 585(2):115-30.PubMed ID17103419 |
Method | TPM-Individual molecules of a specifically biotinated (RNA in this case) polymerase derivative were tagged an avidin-coated polystyrene beads enzyme movement along a surface-linked DNA molecule was monitored by observing changes in bead Brownian motion by light microscopy. |
Entered by | Uri M |
ID | 103168 |