| Value |
0.81
kb/min
Range: ±0.07 kb/min
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Zenklusen D, Larson DR, Singer RH. Single-RNA counting reveals alternative modes of gene expression in yeast.Nat Struct Mol Biol. 2008 Dec15(12):1263-71. p.1269 left column 2nd paragraphPubMed ID19011635
|
| Method |
Abstract:"Using an in situ hybridization [FISH] approach that detects single mRNA molecules, [investigators] measured mRNA abundance and transcriptional activity within single Saccharomyces cerevisiae cells." See fig. 8a: Synthesis time was plotted against the length of the gene. The slope of the line gives (polymerase speed)^-1 |
| Comments |
"The distribution of nascent chains further implies a synthesis time uniquely determined from the fit. If plotted against the effective length of the gene, the inverse slope provides the average speed of RNA polymerase: 0.81±0.07 kb minute-1 (Fig. 8a)." "The elongation speed is slower than the elongation speed measured from a Gal promoter–driven gene, measured at 2 kb/min. A velocity of 2 kb/min (BNID 103016), however, does not fit [investigators'] data, suggesting that different elongation speeds exist for different classes of gene." |
| Entered by |
Uri M |
| ID |
103012 |