Percent of ribosomes associated with mRNA

Value 85 %
Organism Budding yeast Saccharomyces cerevisiae
Reference Zenklusen D, Larson DR, Singer RH. Single-RNA counting reveals alternative modes of gene expression in yeast.Nat Struct Mol Biol. 2008 Dec15(12):1263-71.PubMed ID19011635
Method an in situ hybridization (FISH) approach that detects single mRNA molecules, mRNA abundance and transcriptional activity within single Saccharomyces cerevisiae cells was measured. See fig. 8a: Synthesis time was plotted against the length of the gene. The slope of the line gives (polymerase speed)^-1
Comments There are 2*10^5 ribosomes in a yeast cell (Bion 100267). This value is similar to (Arava 2003, pubmed 12660367) which found 71 percent of ribosomes associated with mRNA for 5701 genes
Entered by Uri M
ID 103025