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Inner aqueous volume of a synaptic vesicle

Value	2E-05 µm^3
Organism	Rat Rattus norvegicus
Reference	Takamori S et al., Molecular anatomy of a trafficking organelle. Cell. 2006 Nov 17 127(4):831-46. DOI: 10.1016/j.cell.2006.10.030 p.839 table 2 & p.843 right column 2nd paragraphPubMed ID17110340
Method	P.836 right column 2nd paragraph: "To measure vesicle volume, [investigators] employed cryoelectron microscopy of shock-frozen and unstained vesicle samples imaged directly in vitrified ice."
Comments	P.843 right column 2nd paragraph: "SVs contain neurotransmitter transporters that are fueled by a proton electrochemical gradient (reviewed in Ahnert-Hilger et al., 2003 ). [Researchers'] results show that vesicles contain approximately ten copies of either VGLUT1 or VGLUT2, and it is likely that the copy number for VGAT, VAChT, or VMAT1/2 is similar on the respective vesicles. With a membrane thickness of 4 nm, the inner volume of an average vesicle is 19.86×10^-21l, equal to 1790 molecules of glutamate [BNID 102777] assuming a concentration of 150 mM (Table 2)."
Entered by	Uri M
ID	102776