| Value |
10
%
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Zinoni F, Birkmann A, Leinfelder W, Böck A. Cotranslational insertion of selenocysteine into formate dehydrogenase from Escherichia coli directed by a UGA codon. Proc Natl Acad Sci U S A. 1987 May84(10):3156-60.PubMed ID3033637
|
| Method |
By localized mutagenesis, the UGA codon was converted into serine (UCA) and cysteine (UGC and UGU) codons. Each mutation relieved the selenium dependency of fdhF mRNA translation. |
| Comments |
in-frame UGA codon at amino acid position 140 that is translated to selenocysteine. Alteration of codon to code cysteine abolished selenocysteine incorporation |
| Entered by |
Uri M |
| ID |
102614 |