Length of siRNA
| Range | 21-23 nucleotides |
|---|---|
| Organism | Human Homo sapiens |
| Reference | Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 2001 May 24411(6836):494-8.PubMed ID11373684 |
| Method | Western blotting and immunofluorescence microscopy. The firefly (Pp-luc) and sea pansy (Rr-luc) luciferase reporter-gene plasmids |
| Comments | RNAi is ATP dependent yet uncoupled from mRNA translation. |
| Entered by | Uri M |
| ID | 102484 |