| Value |
3.74
µm
Range: ±0.15 µm
|
| Organism |
Fission yeast Schizosaccharomyces pombe |
| Reference |
Wu JQ, Pollard TD. Counting cytokinesis proteins globally and locally in fission yeast. Science. 2005 Oct 14 310(5746) Supporting Online Material for reference at www.sciencemag.org/cgi/content/full/310/5746/310/DC1 p.5 bottom paragraphPubMed ID16224022
|
| Method |
Researchers used the cell area (A) measured from DIC images to calculate cell volume. Researchers used fluorescence microscopy to measure global and local concentrations of 28 cytoskeletal and signaling proteins fused to yellow fluorescent protein (YFP) in the fission yeast Schizosaccharomyces pombe. Native promoters controlled the expression of these functional YFP fusion proteins. Fluorescence measured by microscopy or flow cytometry was directly proportional to protein concentration measured by quantitative immunoblotting. Live cells in growth chambers (S2) were observed with a Plan-Apo 100X/1.4 NA
objective on an UltraView RS spinning-disk confocal microscope (PerkinElmer Life and
Analytical Sciences, Boston, MA) using a 514-nm argon ion laser (488 nm for strain
JW1213 mid2-mEGFP) and emission filter 525 to 625 nm (peak at 575 nm). The
refractive index of the immersion oil is 1.52. Fluorescence and differential-interference-
contrast (DIC) images were collected with an ORCA-ER cooled CCD camera. The DIC
images are compromised by the Piezo Z objective collar that increases the distance
between the objective and the Wollaston prism and by their passing through the spinning
pinhole disc." |
| Comments |
"S. pombe cells have a regular rod shape with a constant diameter of D = 3.74 ± 0.15 µm
(N = 2600 cells)." According to Nurse, PMID 7949418 p. 613 left column 2nd paragraph, diameter is ~3µm |
| Entered by |
Uri M |
| ID |
102277 |