| Value |
278
fg
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Loferer-Krössbacher, M., Klima, J., Psenner, R. Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. 1998. Appl Environ Microbiol. 64(2). pp.688-94.PubMed ID9464409
|
| Primary Source |
Heldal, M., Norland, S., Tumyr, O. X-ray microanalytic method for measurement of dry matter and elemental content of individual bacteria. 1985. Appl Environ Microbiol. 50(5):1251-1257PubMed ID3911897
|
| Method |
A method for the determination of dry matter and elemental content of individual bacterial cells is described. The method is based on energy-dispersive X-ray microanalysis in a transmission electron microscope. A theory for area correction of intensity is developed. |
| Comments |
grown in batch culture with minimal medium. Grown in batch culture with minimal medium containing glucose (8mM) as the growth limiting nutrient. Researchers consider the main sources of error in the X-ray microanalytical method to be the mass loss caused by the electron beam (19) and varying beam current from one analysis to another. For the method proposed in primaary source, mass loss is of crucial importance for two reasons. They use the X-ray microanalytical method for estimation of dry matter content of single particles, and they use the same analysis for quantification of specific elements based on the Hall method (Primary source ref 15). |
| Entered by |
Phil Mongiovi |
| ID |
102242 |