Range |
exponentially growing cells 489fg and 710fg: stationary cells 179 fg and 180 fg fg
|
Organism |
Bacteria Escherichia coli |
Reference |
Feijó Delgado et al., Intracellular water exchange for measuring the dry mass, water mass and changes in chemical composition of living cells. PLoS One. 2013 Jul 2 8(7):e67590. doi: 10.1371/journal.pone.0067590 p.5 right column top paragraphPubMed ID23844039
|
Primary Source |
[5] Loferer-Kro¨ßsbacher M, Klima J, Psenner R (1998) Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. Appl Environ Microbiol 64: 688–694. [7] Fagerbakke KM, Heldal M, Norland S (1996) Content of carbon, nitrogen, oxygen, sulfur and phosphorus in native aquatic and cultured bacteria. Aquat Microb Ecol 10: 15–27. doi:10.3354/ame010015.PubMed ID9464409
|
Method |
Transmission electron microscopy |
Comments |
"[Researchers'] dry mass results [203fg BNID 111210] are consistent with previous reported
measurements of the described single-cell or bulk methods. For
instance, two TEM-based studies found median E. coli dry masses
of 489 fg and 710 fg for exponentially growing cells and 179 fg
and 180 fg for stationary ones [primary sources]. [Researchers] report median masses of
725 fg and 179 fg, respectively, pooling technical replicates shown
in Figure 2." |
Entered by |
Uri M |
ID |
111211 |