| Value |
4
molecules
|
| Organism |
Bacteria Escherichia coli |
| Reference |
X. Sunney Xie, Paul J. Choi, Gene-Wei Li, Nam Ki Lee, and Giuseppe Lia, 'Single-Molecule Approach to Molecular Biology in Living Bacterial Cells', Annu. Rev. Biophys. 2008. 37:p.433 caption of figure 7bPubMed ID18573089
|
| Primary Source |
Yu J, Xiao J, Ren X, Lao K, Xie XS. 2006. Probing gene expression in cells, one molecule at a time. Science 311:1600–3PubMed ID16543458
|
| Method |
"observation of real-time production of single protein molecules in individual cells. A fusion protein of a fast-maturing yellow fluorescent protein (YFP) and a membrane-targeting peptide was expressed under a repressed condition" |
| Comments |
"Gene expression occurs in bursts, with each burst originating from a stochastically transcribed single messenger RNA molecule. Protein copy numbers in the bursts follow a geometric distribution" |
| Entered by |
Uri M |
| ID |
102044 |