Unbinding time of LacI transcription factor to DNA
| Range | 1-5 msec |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Jie Xiao, Johan Elf, Gene-Wei Li, Ji Yu and X. Sunney Xie, "Imaging Gene Expression in Living Cells at the Single-Molecule Level" in "Single Molecule Techniques A Laboratory Manual" Edited by Paul R. Selvin and Taekjip Ha, Chapter 7, p. 158, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 2008. |
| Method | in vivo stroboscopic illumination with intense laser exposure for a very short duration (0.3 msec), 568-nm laser line from the Innova Sabre (Coherent) mixed-gas laser. |
| Comments | single red FPs tdTomato in the E. coli cytoplasm |
| Entered by | Uri M |
| ID | 102007 |