Fraction of time repressor is nonspecifically bound to DNA

Value 90 %
Organism Bacteria Escherichia coli
Reference X. Sunney Xie, Paul J. Choi, Gene-Wei Li, Nam Ki Lee, and Giuseppe Lia, 'Single-Molecule Approach to Molecular Biology in Living Bacterial Cells', Annu. Rev. Biophys. 2008. 37:p.427PubMed ID18573089
Primary Source Elf J, Li G, Xie XS. 2007. Probing transcription factor dynamics at the single-molecule level in a living cell. Science 316:1191–94PubMed ID17525339
Method from formula Deff = D3D(1 - F ) + FD1D/3,where F is above property and that the second term is negligible. Deff=0.4µm^2/sec, D3D=3µm^2/sec (Bions 102038,102039)
Comments This suggests that in searching for the operator, a lac repressor spends ~90% of its time nonspeci?cally bound to and diffusing along DNA
Entered by Uri M
ID 102040