Time for fluorescence protein FP to diffuse throughout entire cytoplasm
| Value | 100 msec |
|---|---|
| Organism | Bacteria |
| Reference | Jie Xiao, Johan Elf, Gene-Wei Li, Ji Yu and X. Sunney Xie, "Imaging Gene Expression in Living Cells at the Single-Molecule Level" in "Single Molecule Techniques A Laboratory Manual" Edited by Paul R. Selvin and Taekjip Ha, Chapter 7, p. 150, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 2008. |
| Comments | FP fluorescence is spread over the entire cell and overwhelmed by the autofluorescence background. This problem can be overcome by confining the FP spatially in the cell so that its emitted photons are collected from a diffraction-limited spot instead of the entire cell. |
| Entered by | Uri M |
| ID | 102001 |