GFP brightening time of red fluorescence after photoactivation

Value ~0.7 sec
Organism Bacteria Escherichia coli
Reference Elowitz MB, Surette MG, Wolf PE, Stock J, Leibler S. Photoactivation turns green fluorescent protein red. Curr Biol. 1997 Oct 1 7(10):809-12. p. 810 left columnPubMed ID9368766
Method Researchers exposed GFP in individual bacterial cells to brief (~30 ms) pulses of 488 nm light, and monitored the total intensity of cellular red fluorescence as a function of time after the end of the photoactivating pulse. The result is a slow brightening with a characteristic (1/e) timescale of about 0.7 sec.
Comments 1/e time scale, GFP 'enhanced brightness' variants
Entered by Uri M
ID 101996