| Range |
14.02-15.21 µm
|
| Organism |
Chinese hamster ovary (CHO) |
| Reference |
Han et al. Cultivation of Recombinant Chinese Hamster Ovary Cells Grown as Suspended Aggregates in Stirred Vessels. 2006. J Biosci Bioeng. 102(5):430-5. p.432 left column bottom paragraphPubMed ID17189170
|
| Method |
P.431 right column 2nd paragraph: "Aggregate size distribution assay: The mean diameter of the G6-1 cell aggregates in the spinner flasks and bioreactor was determined by the image analysis of 40 to 60 aggregates, since the aggregates were essentially spherical in shape. The images were captured directly from an eyepiece reticule and an inverted light microscope (Eclipse TS300 Nikon, Kanagawa) using Image-Pro Plus analysis software (Media Cybernetics, Silver Spring, MD, USA) and a Pixera Pro 150ES digital camera (Pixera, Los Gatos, CA, USA)." |
| Comments |
P.432 left column bottom paragraph: "Size distribution of cell aggregates: The distribution of G6-1 cell aggregates size was determined by measuring the mean diameter of the aggregates, since the aggregates were essentially spherical in shape. The mean diameter of the cell aggregates reflecting aggregate size increased with culture time in the chitosan-added suspension culture, shifting from 65 to 163 μm after 2 and 9 d of culture On the contrary, the mean diameter of the cells decreased from 15.21 μm at 1 d to 14.02 μm at 9 d (Fig. 2). As no distinct change in the number of the aggregates was observed over a period of 9 d (data not show), it is possible to conclude that the increase in the mean diameter of the aggregates was contributed to the proliferation of the G6-1 cells. And the decrease in the mean cell diameter can be explained by the more compact of the cell aggregates. The size distribution for suspended G6-1 cell aggregates after 9 d of cultivation in the chitosan-added suspension culture in spinner flasks is presented in Fig. 3. A large fraction of the aggregates (roughly 72%) were between 100 and 200 μm in diameter. The fractions of aggregates that were below 100 and above 200 μm in diameter were about 17% and 11%, respectively (Fig. 3)." |
| Entered by |
Phil Mongiovi |
| ID |
101901 |